Human Insulin ELISA kit is an immunosorbent assay kit used for the quantitative measurement of Insulin and Proinsulin in serum, plasma, cell culture supernatants, and pee. This kit was used to ascertain individual insulin and proinsulin concentration in serum and human adrenal microvascular endothelial cells that were isolated from individual visceral fat of nondiabetic patients.
Human Insulin ELISA Kit has been utilized to assess the levels of insulin and proinsulin from the transgenic mice, to quantify insulin in multiple sclerosis. The Human Insulin ELISA equipment quantifies human insulin. The assay will recognize both the natural and recombinant insulin.
In this process, the Individual insulin solid-phase sandwich ELISA is intended to assess the total amount of the object bound involving a paired antibody pair. A target-specific antibody was pre-coated from the wells of their provided microplate. The sandwich is formed by the inclusion of this next antibody, a substrate solution is added.
Each lot of the ELISA kit is quality tested for standards like sensitivity, specificity, accuracy, and consistency. Insulin is a peptide hormone secreted by beta cells of the pancreatic islets in humans. It regulates protein, carbohydrate, and lipid metabolism by improving membrane transport of glucose, amino acids, and specific ions.
Additionally, it promotes glycogen storage, the creation of sugars, and the absorption of fats and nucleic acids. Insulin is an amino acid polypeptide product formed by a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains which are covalently connected via two disulfide bonds. Insulin deficiency leads to diabetes mellitus and can also be found in tumors of b-cell sources like insulinoma.